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Literature summary extracted from

  • Rui, Z.; Petrickova, K.; Skanta, F.; Pospisil, S.; Yang, Y.; Chen, C.Y.; Tsai, S.F.; Floss, H.G.; Petricek, M.; Yu, T.W.
    Biochemical and genetic insights into asukamycin biosynthesis (2010), J. Biol. Chem., 285, 24915-24924.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.215 protein AsuE2 endogenous protein AsuE2, UniProt-ID D7P5W0, is necessary for the full catalytic function of AsuE1 as a two-component flavoprotein hydroxylase. AsuE2 is a homologue of the flavin reductase PheA2, which recycles oxidized FADox and provides FADred to its hydroxylase partner PheA1 in the phenol hydroxylation in Bacillus thermoglucosidasius A7 Streptomyces nodosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.215 cloning of asukamycin biosynthetic gene cluster, including gene asuE1 encoding the enzyme, recombinant expression in Escherichia coli strain BW25113/pIJ790, heterologous expression of the complete gene cluster in the asukamycin-nonproducing Streptomyces lividans strain K4-114 Streptomyces nodosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.215 protoasukamycin + NADH + H+ + O2 Streptomyces nodosus
-
 4-hydroxyprotoasukamycin + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.215 Streptomyces nodosus D7P5V0 subsp. asukaensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.215 protoasukamycin + NADH + H+ + O2
-
 Streptomyces nodosus 4-hydroxyprotoasukamycin + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.215 asuE1
-
 Streptomyces nodosus
1.14.13.215 protoasukamycin 4-hydroxylase
-
 Streptomyces nodosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.215 FAD FAD is recycled by enzyme AsuE2 Streptomyces nodosus
1.14.13.215 NADH
-
 Streptomyces nodosus

General Information

EC Number General Information Comment Organism
1.14.13.215 metabolism in the asukamycin metabolism, enzymes AsuE1 and AsuE2 catalyze the conversion of protoasukamycin to 4-hydroxyprotoasukamycin, which is epoxidized at C5-C6 by enzyme AsuE3 to the final product, asukamycin. Synthesis and structures of asukamycin and related metabolites, overview Streptomyces nodosus